|
KMID : 1025520040460030495
|
|
Journal of Animal Science and Technology
2004 Volume.46 No. 3 p.495 ~ p.502
|
|
|
Expression of Recombinant Intimin of Escherichia coli O157:H7 and its Effect of Immune Response
|
|
Kim D.-G.
Lee S. R.
Kim Jung-Woo
|
|
|
Abstract
|
|
|
|
Intimin, the product of eae gene in EHEC O157:H7, is required for intimate adherence. In this study, the C-terminal region(281 amino acids) of the EHEC O157:H7 intimin were expressed as a protein fusion with (His)6, which was used to raise antiserum in rabbits. The antiserum reacted in western blot with a 94kDa outer membrane protein of EHEC O157:H7. It was observed that the antibody titers both in egg yolk and serum appeared in 2～4 weeks after immunization with fusion protein. At the time of 8 weeks, the titre of egg yolk was found to be higher than that of sera. According to the results of neutralization test, chicken egg-yolk antibody(IgY) against the recombinant intimin strongly reacted to EHEC O157:H7. We conclude that a truncated recombinant intimin could be used as an immunogen to elicit antibody(IgY) against O157:H7.
|
|
|
KEYWORD
|
|
|
EHEC O157:H7, Recombinant intimin, IgY
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|